Single Cell RNA Sequencing - replicates pooling and barcoding
Hi all, I'm sorry if this question has been answered in this forum. I am planning a single cell RNA-seq experiment where I will compare three conditions starting from cells cultured in a dish (human pluripotent stem cells derived cells). I will also include a negative control.
My question is: is it possible to pool the replicates for each condition, and barcode it individually to retrospectively identify the individual replicate? I would like to pool replicates to contain costs allowing me to include more conditions instead.
Thank you very much for your help!
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Bolesaem •
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Which single cell sequencing method/technology are you planning to use?
Thanks for replying! Well, the question for me now is: what type of sequencing method would allow me to do it?
I have mostly seen single cell data being sequenced using Illumina (10x, drop-seq, fluidigm etc).