Single Cell RNA Sequencing - replicates pooling and barcoding

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4.7 years ago

Bolesaem ▴ 10

Hi all, I'm sorry if this question has been answered in this forum. I am planning a single cell RNA-seq experiment where I will compare three conditions starting from cells cultured in a dish (human pluripotent stem cells derived cells). I will also include a negative control.

My question is: is it possible to pool the replicates for each condition, and barcode it individually to retrospectively identify the individual replicate? I would like to pool replicates to contain costs allowing me to include more conditions instead.

Thank you very much for your help!

scRNA-seq barcode replicates pooling • 1.6k views

ADD COMMENT • link 4.7 years ago by Bolesaem ▴ 10

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Which single cell sequencing method/technology are you planning to use?

ADD REPLY • link 4.7 years ago by GenoMax 141k

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Thanks for replying! Well, the question for me now is: what type of sequencing method would allow me to do it?

ADD REPLY • link 4.7 years ago by Bolesaem ▴ 10

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I have mostly seen single cell data being sequenced using Illumina (10x, drop-seq, fluidigm etc).

ADD REPLY • link 4.7 years ago by GenoMax 141k

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