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perseus expert assist

Need some advice on my current work. I need experts to look at my perseus workflow. It's my first time to work on this. Please assist, thanks

gene
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updated by Ram Ram • written by BabySteps • BabySteps

good day and thanks for the reply and for the attention

i have 4 sets TMT-labelled mass spec raw files from paired disease-normal tissue, and 2 extra channels as internal referernce for normalization. And I have their average abundance, abundance ratio, and other quantitation from each labelled sample - 126, 127D, 127N, 128-D, 128-N, 129-D, 129-N, 130-D, 130-N, and 131

I want to quantify each protein from each patient one at a timedf, an determine those up and down-regualted. My question is how do I make use of the 2 extra channels for in doin quantitation. Thank you very much for your recommendation and time.

Babysteps

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written by BabySteps • BabySteps

How can we help you with your workflow if you don't show it?

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written by WouterDeCoster WouterDeCoster

good day and thanks for the reply and for the attention

i have 4 sets TMT-labelled mass spec raw files from paired disease-normal tissue, and 2 extra channels as internal referernce for normalization. And I have their average abundance, abundance ratio, and other quantitation from each labelled sample - 126, 127D, 127N, 128-D, 128-N, 129-D, 129-N, 130-D, 130-N, and 131

I want to quantify each protein from each patient one at a timedf, an determine those up and down-regualted. My question is how do I make use of the 2 extra channels for in doin quantitation. Thank you very much for your time.

Babysteps

• 0 views • link
written by BabySteps • BabySteps

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