Hello everyone
I am using DiffBind Bio-conductor for differential binding analysis. I have four replicates of same experiment so the Condition column is same for all the replicates, while getting the contrast in R (i.e My_data <- dba.contrat(My_data, categories = DBA_CONDITION) ) I am getting warning :
Warning message: No contrasts added. Perhaps try more categories, or lower value for minMembers.
I tried changing minMemebers and also the categories but still the same error is coming. Please Help
Thank you...
•
written
by
chaudharyc61 •
1 answer
What are the sample groups that you are trying to find the difference between? If all four samples are replicates of the same sample type, there is nothing to contrast.
Another way to look at this: if all the samples have the same values for the Condition, what other attribute (Tissue, Factor, Treatment) do they have different values for?
Log in to answer this question.
-
Diffbind and DESeq2 comparison for differential sites
written by Ankit
-
Multi-factor designs in DiffBind
written by g.persic1991 •
-
DiffBind error: No contrasts added. There must be at least two sample groups with at least three re…
written by estel_ferre •
-
Strange results from Diffbind-Deseq2 differential peak binding of cfChip-seq with blocking
written by Oladele •
-
DiffBind - low number of significant peaks for one contrast
written by CB •
-
DiffBind 3.2 error
written by Huang Zhihao •
-
How to make complex contrasts in DiffBind dba.contrast
written by Bioinformatist Newbie
-
DiffBind without replicates
written by zhangdengwei
-
DiffBind: Error in `.rowNamesDF<-`(x, value = value) : invalid 'row.names' length
written by msimmer92
-
R's DiffBind. Error in pv.DBAplotVolcano(DBA, contrast = contrast, method = method,: object 'sigSit…
written by msimmer92
