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filter transcript isoforms with low support

Hi, I need to filter transcript isoforms of my de novo assembled transcriptome with low support by removing isoforms minor of a percentual of the Trinity gene subcomponent read support. (in my case 0.01%) What is the correct strategy? I never made that. Thanks

rna-seq filter assembly

1 answer

It's quite common to filter transcripts on both expression (read counts) and the isoform fraction (fraction of gene expression originating from each isoform) so sounds like a good plan. Just make sure that you calculate your gene expression before doing that filtering (aka the transcript removal should not effect the gene expression).

Thanks a lot. Can you indicate to me some software to do it?

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