Dear all, I am trying to do methylation analysis on TCGA data using TCGAbiolinks.

Since I only wanted to use a limited subset of samples, I used their TCGA codes in:

query.met.normal = GDCquery(project = "TCGA-THCA",legacy = TRUE,data.category= "DNA methylation",

platform = "Illumina Human Methylation 450",

barcode = c("TCGA-EM-A3ST-11A-11D-A231-05" ,"TCGA-FY-A3TY-11A-12D-A231-05" , ...)).

After downloading data which were saved in "C:\Users\Administrator\Documents\GDCdata\TCGA-THCA\legacy\DNA_Methylation\Methylation_Beta_Value", I used :

met.normal.450 = GDCprepare(query = query.met.normal,save= TRUE,save.filename = "normalDNAmet450k.rda" ,summarizedExperiment = TRUE),

however I got this error:

Error in rbind(deparse.level,...) numbers of columns of arguments do not match.

Can you guide me to solve this problem?

Kind regards

Nazanin