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How to get DNA sequences of multiple peptide sequences (25600) in R or Python

Hi all,

I have a file of 25600 peptide sequences:

>sp

YYRITYGETGGNSPVQEFTVPGSK
>sp

YYTEFPTVLDITAEDPSK
>sp

YYTGVVNNNEMVALQR
>sp

YYTLNGSK
>sp

YYTSASGDEMVSLK
>sp

YYTYLIMNK
>sp

YYVTIIDAPGHR
>sp

I used EMBOSS Backtranseq to convert the peptide into DNA sequences, but it will only take 500 peptide sequences. Is there any way on R or Python where I can run a script that will convert the peptide sequences into DNA sequences please?

I new to this area of bioinformatics, so any help would be greatly appreciated.

Many Thanks,

Ishack

r dna sequencing python

Hello ishackm!

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