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Tophat fusion error
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15 months ago
baurumon • 10

Hi, I have run Tophat fusion with paired-end RNA-Seq data, but was unsuccessful. The error says running 'long_spanning_reads' . then i split fastq file and run again. unfortunately it is showing same error. could anyone help me in this process!!! My command was -- tophat2 -o tophat_fusion -p 15 --fusion-search --keep-fasta-order --bowtie1 --no-coverage-search index_file unmapped1.fastq unmapped2.fastq

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