Where could I get raw data of illumina DNA sequncing? in BAM,SAM or fastQ format. Is there any kind of online free database for such a thing.
for optimization and testing purposes, I need data of both, good and bad quality to feed it into a pipeline I am working on.
2 answers
NCBI SRA is the main repository for raw sequence data. It will be in fastq format. There may be some aligned data available there but that would be in much smaller numbers.
The data is generally replicated at EBI-ENA and DNA bank of Japan (DDBJ).
Note: Most of the data should be available freely. Certain types of data (e.g. human datasets from some projects) require additional approval.
Please see for suggestions:
Fast download of FASTQ files from the European Nucleotide Archive (ENA)
sra-explorer : find SRA and FastQ download URLs in a couple of clicks
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