Thanks a lot Dave! I will check it out
Assembler for only nanopore data
Hi Everyone! Does anyone know about an assembler where the input is ONLY nanopore data (i.e, no Illumina or any other kind of short reads)? What about using ABySS for assembling only nanopore reads, is it OK? (I know that SPAdes should not be used if I have only nanopore reads)
Thanks a lot!
• 5,906 views
•
link
4 answers
I believe Canu is a popular option. Should work for either nanopore or PacBio long read data.
• 0 views
•
link
• 0 views
•
link
You can try Canu, Flye, Necat, Minimap-miniasm, Ra etc.
• 0 views
•
link
Shasta is very good and fast, provided you have lots of RAM (over 500 GB for many genomes).
• 0 views
•
link
Log in to answer this question.
Have a look here you will probably find what you want.
Edit by GenoMax: Outdated link corrected.
I've seen good reviews of Flye for nanonpore data (I never uded it myself).
Prior the the assembler you may user nanoq for quality control https://github.com/esteinig/nanoq and/or Kraken for contamination detection https://github.com/DerrickWood/kraken https://www.youtube.com/watch?v=n-6pH3EPZDI