RNASeq analysis, FastQC how to solve warning and fail
I do a FastQC for quality control my data but i some situation there are a caution, can anyone tell me how to solve these problems for each situation? Thanks Per base sequence content = FAIL Per sequence GC content = ! Sequence Length Distribution = ! Sequence Duplication Levels = !
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That isn’t enough information for us to go on. It is common for FastQC to fail several tests, and with RNAseq, it’s even more common.