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genome assembly by Masurca
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18 months ago

Hi, I am very new to genomics and currently running MaSurca. Just wanted to know if any polishing steps (pilon or racon) are suggested after assembly? Thank you for assistance in advance.

Sonia

Assembly • 165 views
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I assume, you assemble some sort of long reads mixed with n short read libraries. Depending on your coverage, it might not be necessarily, but I would run it in any case at least once to see how much is polished. Any of the two programs is good, I have worked with pilon, so far.

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I have paired end, mate-pair and pacbio reads. I will go ahead with pilon polishing then. Thanks.

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