I am a beginner in bioinformatics and I am looking for a robust pipeline to identify optimal shRNA targets within a highly variable transposable element …

I know the way MACS2 uses Poisson distribution to find the p value for the enrichment of a signal peak(observed) as compared to the local …

I am assembling a genome using Canu 2.2 with the following commands on a slurm cluster: ``` canu useGrid=false \ -p tf -d tf-pacbio \ …

Hello I was asked to perform an assembly polishment with Racon . The issue is that we are trying to assembly the genome of n …

Hi everyone, I have a datasets where the coverage of input is relatively. Actually many reads were filtered out during deduplication step. When I performed …

Hi to every one, I'm running a pipeline in which i need as an input file Pacbio reads but unfortunately the pacbio reads available have …

I prepare genomic shotgun libraries for sequencing using Single Molecule Real Time (SMRT) sequencing platform produced by PacBio (20X coverage of PacBio reads) coupled with …

When generating secondary analysis data from PacBio sequencing, you can use the SMRT View with your data, but the visual representation does not seem intuitive/friendly …

Hi! When performing genome assembly, it usually need high coverage data, such as more than 50X for pacbio, in my opinion, 10X would be enough …

I used pacBioToCA to correct some long reads LR using illumina short reads SR from the log file there is this info: ``` INPUT_NAME OUTPUT_NAME …