I am using ADVOCATE R package to determine virtual expression from bulk gene expression. I am currently using TCGA-PAAD data. I have checked ADVOCATE manual, I am unable to understand the output generated. My question is how to interpret the output in this case? Can we use ADVOCATE trained data for cancer other than Pancreas?
Here are the output files.
I would probably not apply ADVOCATE to other cancer types than Pancreas. ADVOCATE is (one of many) tools that require a set of "pure" expression profiles (reference profiles) from all cells you want to infer expression from in the bulk data. That also means results will be unreliable if your set of reference profiles is not adequate. In their paper they extract the reference dataset from Pancreas cancers - but it is very likely other cancer types will have another set of reference profiles (even the same cell types will have different profiles) thereby undermining the results. So unless you have a set of reference profiles for you data you should probably not use any such tools.
Could you post a link to the R package?
It is here, Its Github repository.