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Identification of different bacterial strains in mixed population Sequencing

I want to identify different bacteria in a microbial mixed population using non-16Srna regions. I have two questions- 1. Is there any alternative approach out there may be based on consensus sequence or marker genes of different bacteria or anything else. Most of publications are based on 16sRNA based OTU method. 2. Is there a database listing marker genes of each bacterial species.

Thanks

sequencing

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