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HTSeq error while counting reads

Hello Everybody, I am getting this following error while running HTSeq on BAM files generated by STAR:

Error occured when reading beginning of SAM/BAM file. unsigned byte integer is greater than maximum Exception type: OverflowError, raised in libcutils.pyx:50 How do I solve this issue?

rna-seq
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written by makwana.kd • makwana.kd

Please provide much more context for this issue. It's kinda hard to investigate this given the current information

Eg. provide the exact cdmline you're trying to run. Post a glimps of the input files you are using, ... things like that. thx

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written by lieven.sterck lieven.sterck

Hello Lieven.streck,

Thanks for replying. I found that something was not right on the BAM while, so what I did was convert BAM into SAM file and then it through HTSeq, and it worked.

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written by makwana.kd • makwana.kd

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