Hello everyone! I would like to ask adiveces for trascription factor binding site prediction.
My co-supervisor asked me to check if a particular human gene promoter has potential binding site for a TF.
I have tried solving out this problem by coding a matlab code based upon information theory but I have found problems with defining a method in order to determine significance level of my result (since Σf*I values don't give any information by themselfs). I have tried one but it has failed to make positive predictions for a consensus site in promoters chosen as "positive controls". Therefore, I would like to change the approach and looking for some online predictors.
Have you got any advice?
I have tried using JASPAR on Genome Broswer but it seems to be not so much convenient since I am not able to download the analysis (and it means I have to check each TF name on the screen, because I cannot use the search function).
Thank you for your support!
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written
by
Eisuan •
2 answers
If you are looking for an online tool where you can upload a list of genomic coordinates and get the overlapping TF motifs, I would suggest our developed resource called: funMotifs. It also provides tissue-type specific annotations. Alternatively, you could use regulomeDB
However, if you are looking to identify motif instances of a particular TF in a particular sequence then you could use FIMO.
Some answers I've written on searching promoters for TFBS using FIMO:
Adjust for your organism, assembly, and TF databases of interest.
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