How to Calibrate the Ultrametric species phylogeny tree?
I am willing to develop a ultrametric species phylogenetic tree of Prokaryote and Eukaryote species using Bayesian algorithm. I would like to know about the appropriate root calibration value for the tree and as we all know from the previous study the fossil of Archea is around 3.4 Billion years ago and in some of the literature the divergence of Prokaryote and Eukaryotic species is vary from 2.1-2.4 Billion. So, my question is what should I consider for the calibration of the outgroup of tree, is this 3.4 or 2.1 Billion Years Ago? Any suggestion or comment on this will be highly appreciated.
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My question is very simple, for the divergence time estimation hard (3.4 Billion years) or Soft (2.1 Billion Years) bound is required?
Are you sure it is Million years ago and not Billion years ago?
Sorry! Its Billion Year Ago, not million and corrected this is my post.