error in read.maimages {limma}

Question

[594915965]

when I run this code

x <-read.maimages(as.character(sampleInfo[,'RawDataFile']),source="agilent",green.only=TRUE,path=paste(data_dir,'raw',sep='/'))

I got an error:

Error in RG[[a]][, i] <- obj[, columns[[a]]] : 
  被替换的项目不是替换值长度的倍数
  

I have set the directory path also I mentioned all the file names in targets.txt. I also re-download the raw data from GEO database.

please help with this.

Thank you.

[h.mon]

I suggest you set your R environment language to English when posting at an international forum. Apparently (Google translate), the error message is:

number of items to replace is not a multiple of replacement length

[594915965]

Sorry,I tried to set Rstudio environment language to English but failed. The error is always in Chinese, I don't know how to change it. I use Linux (Debian),can u help me?Thanks!

Answer 1

[Gordon Smyth]

read.maimages requires all the Agilent data files to correspond to the same set of probes, with one row for each probe. It seems however that your files have different numbers of rows.

[594915965]

Yup ! I found one target.txt is different. When I delete this file, it works!Thank u!