I get two count matrix (tissue A, tissue B) of genes to identity DEGs, the their includes samples technology replication and biological replication.
To get DEGs:
However, if here no technology replications of each sample, I can directly to do compare. So I'm going to get average of technology replications to represent each sample, it's right?
For tissue A, (same for tissue B)
I use tissue A matrix to draw PCA, for each sample, their technology replications shows outliers, but how to quantitative identification them, is their some ways, so remove the outliers to calculation average?
Any views will be much appreciated!