Hi,

I got error while running OTU picking for ITS data. I am woking on HPC server. How I can set BLASTMAT environment variable?

RuntimeError: BLAST cannot run if the BLASTMAT environment variable is not set.

Usually, the BLASTMAT environment variable points to the NCBI data directory,

which contains matrices like PAM30 and PAM70, etc.

Alternatively, you may create a .ncbirc file to define these variables.

From help file:

2) Create a .ncbirc file. In order for Standalone BLAST to operate, you

have will need to have a .ncbirc file that contains the following lines:

[NCBI]

Data="path/data/"

Where "path/data/" is the path to the location of the Standalone BLAST

"data" subdirectory. For Example:

Data=/root/blast/data

The data subdirectory should automatically appear in the directory where

the downloaded file was extracted. Please note that in many cases it may

be necessary to delimit the entire path including the machine name and

or the net work you are located on. Your systems administrator can help

you if you do not know the entire path to the data subdirectory.

Make sure that your .ncbirc file is either in the directory that you

call the Standalone BLAST program from or in your root directory.

Thanks