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How to generate a family level phylogeny?

Hi all,

I want to generate a 16S family level phylogenetic tree of a bacteria family, and include on the sequence I have to see where it falls within the family. I know the SILVA database has a lot of 16S sequences, that I downloaded. But making a tree out of all those sequences requires much space, that my laptop doesn't have. Also I don't need thousands of sequences. Just enough to have representative sequences to cover the whole family.

So how should I go about this?

Thanks in advance :)

phylogeny 16s

Do you have any access to hardware with more power than your laptop?

Check out those cloud services such as aws(amazon web services). You pay for it base on your demand. A 16g memory for 5 hours only takes you around $20. Plus, have you checked the qiime2's phylogenic tree function? That actually doesn't use a ton of resources.

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