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RNA-seq cDNA Samples

Hi all,

I am preparing samples to send for RNA-sequencing. I've transformed a majority of my samples to cDNA, keeping only a few microliters of highly concentrated RNA for QC analysis, as I had concerns over the RNA stability. However, on the sequencing page, it seems as if most samples are sent in RNA form. Has anyone successfully sent cDNA samples for RNA-sequencing?

Thanks in advance.

rna-seq
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written by michaell • michaell

Hello michaell!

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written by GenoMax GenoMax

With exception of RNA sequencing done directly using Oxford Nanopore technology, all other RNA has to be converted to DNA before it gets sequenced. Even if you happen to submit RNA, the provider will convert to DNA when making libraries.

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written by GenoMax GenoMax

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