thanks a lot, Kevin.
Dear all,
I'm doing a project which I have to use two separate project on GEO, one of them is Agilent array and second is RNA-Seq.
I've searched a lot, but there were no straightforward answer to my question.
Because both condition samples are not available in a single project I have to use different studies.
Is there anyway to do DEG analysis based on these both platform? ( please share your link or code)
Thanks a lot.
1 answer
There is no straightforward answer for this because it is not a recommend procedure. I have provided various answers in relation to this topic:
Microarray and RNA-seq
- Combine microarray and RNA-seq gene expression for differentially expression analysis
- Compare Microarray with RNA-Seq
Microarray and microarray
- A: How to integrate multiple data sets from microarray platform prior meta-analysis
- How to merge different microarray data sets from different platforms (not for differential expression analysis)?
Your best option is to process them independently and then perform a 'meta analysis' of the results. You can also do things like correlate the fold-changes, as I did here with RNA-seq and proteomics:
You could also perform gene enrichment and pathway analysis on the results and then cross-compare.
Kevin
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