So I have 3 conditions WT HET and KO. For each i extracted tumors from the colon. Colon are directional (intestine, mid, anus) aka proximal mid and distal colon. So my WT has tumors in all 3 sections, both HET and KO loose the proximal tumors and my KO is enriched in the mid section while losing some of the distal tumors.

I am new to bioinformatics and R, but the data is clearly not normally distributed. I created a violin plot but box plots seem to only focus on the median hence i want a way to compute significance/enrichement for each section of the colon.

I could always split the data into 3 parts then do a t.test but i dont know if there is a better way to do it. Preferably represent it on a violin plot.

Thanks you