I am using sratoolkit.2.9.2.ubuntu64. I tried downloading RNA-seq data with the command prefetch ERR315616. (eukaryote data)
I got the error message
2018-12-17T05:51:35 prefetch.2.8.2 sys: libs/kns/unix/syssock.c:606:KSocketTimedRead: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed )
2018-12-17T05:51:35 prefetch.2.8.2 int: libs/kns/unix/syssock.c:606:KSocketTimedRead: timeout exhausted while reading file within network system module - �
2018-12-17T05:51:35 prefetch.2.8.2: 1) failed to download ERR315616.
But I am able to download influenza genome with the same command prefetch SRR8292123.
Please help and thanks in advance.
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written
by
kousi31 •
2 answers
The data are backed up at the European Nucleotide Archive (ENA). You can get them from there in fastq format. For batch downloads, also see my tutorial Fast download of FASTQ files and metadata from the European Nucleotide Archive (ENA).
This may be a temporary system outage, see: https://github.com/ncbi/sra-tools/issues/84.
Otherwise, based on the timeout and failed SSL handshake I wouls suspect a firewall connection issue. Do you normally connect via a proxy on your network?
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For me it worked (with sra toolkit 2.8.2):
So my guess is that something is not installed or not working properly on your machine.