converting duplication rate to library complexity?

Hello, Does anyone know a way to convert duplication rate and number of reads to library complexity in the form of how many reads are available to/should be sequenced?

let's say I have 600 million reads and have 70% duplication rate. is there a way to predict my duplication rate were i to sequence 300 million reads?

Thanks!

sequencing

• 1,351 views • link

updated 7.7 years ago by ATpoint

• written 7.7 years ago by euge.eugenio •

1 answer

Check out PreSeq.

• 1 views • link

written 7.7 years ago by ATpoint

More posts like this

Understanding Discrepancies in Deduplication Rates Between Forward and Reverse Reads in Metagenomic…
written 2.1 years ago by Stiofan •

Hello, I am working with Whole Shotgun Metagenomic data, specifically paired-end reads, where each sample typically has a read depth of approximately 50-60 million reads. …
Minimum reads per sample
written 2.5 years ago by rico07 •

Many of the library prep kits provide information on the typical number of reads required for analysis. I know this can depend on the type …
Clarification on sequence duplication levels and Qualimap estimated duplication rate
written 3.0 years ago by keremozdel9 •

Hello, I'm working with illumina paired-end reads. I have merged the data of 2 different runs of the same sample, in order to increase coverage …
Reads vs library size for DEA
written 4.0 years ago by backfish •

Hey, for robust differential expression analysis (DEA) many factors need to be considert (number of replicates, genome complexity etc.). One important parameter is the number …
distinguishing PCR and optical duplicates
written 4.1 years ago by evoecogen •

Hello, Mammalian samples were sequenced using Illumina NovaSeq. FastQC reported that 28-35% of reads were duplicates. I wanted to know if the duplication happened at …
unequal fastq files
written 7.2 years ago by sagardesai91

Hello everyone, I have in-house sequenced raw transcriptome data. The issue is, that the number of reads for different samples are varying by a huge …
Do I need to mark duplicates for sequencing data using multiplex PCR to prepare library?
written 8.1 years ago by MatthewP

Hello. I have some **mtDNA** sequencing data using multiplex PCR to prepare library, which means using many primers to 'copy' whole mtDNA from whole genome …
Normalize coverage to spike-in chromatin
written 8.1 years ago by goodez

I have a number of samples that were sequenced (human cells) under some different conditions. All samples received the same 10% spike-in of mouse chromatin. …
How To Check For The Saturation Of The Library ?
written 12.4 years ago by Ashutosh Pandey

Dear All, It may be a trivial question but what would be a best way to know if resequencing of a transcriptomic library at a …
Tool Or Software To Determing Tag Redundancy (Summary Of Library Complexity)
written 13.0 years ago by k2bhide

Hello all, This is Ketaki Bhide from Purdue University. I tried to use ALEXA-seq to find Summary of library complexity - estimated by tag redundancy …