hi guys i try to use hisat2 aligning the fastq file to the index which i have built before, here is the command line: /home/wyf/miniconda3/bin/hisat2 …

Hello, I am using the below command to align the reads and get bam file: ``` hisat2 -x /hisat/grch38/genome -1 /fastq/output_forward_paired.fq.gz -2 /fastq/output_reverse_paired.fq.gz | samtools …

hi , i am about to using hisat2 in order to align some fastq file and create sam files. i face this err would you …

I have tried to create a hisat2 script loops and am encountering an error message and can't seem to figure where the problem is. Please …

Hello everyone, In order to align the RNA- seq reads to the genome I faced this error: ``` Could not locate a HISAT2 index corresponding …

Hi everyone! I am now working on the wheat genome (Triticum aestivum) with 14.8Gb. Because I am working on my personal device, I tried to …

Hi, I am trying to run Hisat2 on FASTA files. Hisat has always worked for me and I've only just encountered this issue now. It …

Hi all, I'm trying to run hisat2 for aligning an available DRG rna seq using the following command: /data/local/hisat2-2.1.0/hisat2 -p 4 -q --dta --known-splicesite-infile $ANNOT/splicesites.txt …

hello, im going to use hisat2 instead of tophat2. but, i cant solve " `(ERR): hisat2-align exited with value 1` and Error: Encountered internal HISAT2 …

i am using following command to align the reads in hisat2: for my project [memona@farooq hisat2-2.1.0]$ ./hisat2 –p 64 --max-intronlen 10000 –x /data/memona/hisat2-2.1.0/hisat_index -1 /data/memona/SRR959590_A_1P.fq …