Hi, I´m very new to all the NGS processing stuff, but I installed fastq screen successfully and indexed 3 genomes using BWA (human, E. Coli and a custom made one). So far everything went well, but when I try to run fastq screen it gives me this error;
Using '/home/usr/Software/fastq_screen_v0.13.0/bwa/bwa' as BWA path
Skipping DATABASE 'Human' since no BWA index was found at '/home/usr/Genomes/Homo_sapiens/GRCh38_genomic.fa.sa'
despite the path in the config file should be the right one, and I can´t see what else could be the problem (obviously there are all files which should be produced by the bwa index command).
This is the path in the config file for the human genome;
/home/usr/Genomes/Homo_sapiens/GRCh38_genomic
Maybe any of you knows how I could solve this problem?
Say if you are working with test.fasta and that you have provided the following in the config file
## trial1
DATABASE trial1 /path/test.fasta
then, your path should be the absolute location of the the .fasta file along with the index files like this.
test.fasta
test.fasta.amb
test.fasta.ann
test.fasta.bwt
test.fasta.pac
test.fasta.sa
Ok, I found the problem. Vijay pointed me to the soultion; You need to use the fasta file which you indesed, but not the other files made while indexing. Thank you everbody for your hints :)
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FastQ screen is looking for
bwaindexes in/home/franziskasafi/Genomes/Homo_sapiens/directory. Where as you appear to refer to a different directory in config file (not sure what that config is for). Either move/soft link your bwa index files in the directory where FastQ screen is looking for indexes.I´m sorry, I forgot to edit the path in the first hand (because of names). It is all the same path.
Yes, this is how my config-file looks like and the files that are inside of the folders of the indexed genomes.
Please use
ADD COMMENT/ADD REPLYwhen responding to existing posts to keep threads logically organized.Then based on @Vijay's answer the config file path should include names of the directory along with the fasta file.
Ok, I found the problem. Vijay pointed me to the soultion; You need to use the fasta file which you indesed, but not the other files made while indexing. Thank you everbody for your hints :)
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