Demultiplex dual indexed reads considering sequence direction
Hello,
I have a dataset generated by 1D Minion I need to demultiplex. The dataset was dual multiplexed considering the direction. for example a sequence with: Index1+Forward primer & Reverse primer +index2 doesn't belong to the same sample as this sequence: Index2+Forward primer & Reverse primer +index1
Could someone please edit the minibar.py https://github.com/calacademy-research/minibar/blob/master/minibar.py to accommodate the direction option? Or suggest a tool that will work.
Thank you
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salamhabib7 •
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You could check sabre (https://github.com/najoshi/sabre) and cutadapt (https://cutadapt.readthedocs.io/en/stable/guide.html#demultiplexing).