how to find the UMi position?

Hi, In my library, the insert is ligated to an adaptor sequence that contains the first index and the UMI. My question is: the UMI will be in read 1, read 2 or both? in 5' or 3' end? I did a paired end sequencing 150pb. Thank you,

alignment umi

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updated 7.7 years ago by h.mon

• written 7.7 years ago by DNAseq •

Why there is the forum Tag?

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written 7.7 years ago by Santosh Anand

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