bowtie2 unique align reads
I ran bowtie2 in the following way:
bowtie2 -x tdna -U Sample-5-concat+trim-21.fq -S sample5.sam
1792444 reads; of these:
1792444 (100.00%) were unpaired; of these:
1092638 (60.96%) aligned 0 times
698850 (38.99%) aligned exactly 1 time
956 (0.05%) aligned >1 times
39.04% overall alignment rate
How do I get reads which aligned exactly 1 time?
Thank you in advance.
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Hello Ric!
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Thank you it worked.