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novel miRNA identification from sequence

Hi everyone!

I'm looking for a tool that can allow me to predict novel miRNA from their sequences. I tried Mireap but I'm not able to provide the "map.txt" file since I have only the bam and sam from my alignment with Bowtie2. Thanks!!

mirna

can u tell me how did you do ? I have the same quesition

3 answers

Check out the novel miRNA prediction module in sRNABench:

http://bioinfo2.ugr.es:8080/ceUGR/srnabench/

You could try a pipeline we recently developed in our team. Paper is still not published though.

https://github.com/emarmolsanchez/eMIRNA

Use UEA sRNA workbench - miRCAT

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