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Error from snpEff annotate

Hi all,

It is my first time to use snpEff to annotate my VCF file generated from GATK. After software installation and reference genome download, I use the typical command to do annotation as below.

java -jar SnpSift.jar annotate referenceVCFfile.vcf myVCFfile.vcf > variants_annotated.vcf

The first error came out,

**Error        :    Unknown parameter 'myVCFfile.vcf'**.

Could you share the solutions or advice?
Thanks.

snpeff annotate

Hello boymin2020!

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Hello,

what version are you using? What happens if you start java -jar SnpSift.jar annotate without any parameter?

fin swimmer

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