Extracting miRNA data from RNA-Seq data set
Can i extract miRNA data from RNA-Seq data set ? and if possible, can i extract deferentially expressed microRNA's from it ?
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hafiz.talhamalik
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Probably not. Typical RNA-seq library preparation includes a size selection for RNA/cDNA of like 200bp. As short RNAs are (guess what) shorter, whatever signals you pick up will probably be unreliable.
Ok thanks got it...!
Brother #Hafiz.Talhamalik, If you have extracted the miRNAs from RNA-seq data, please mention here your pipeline, I will be really thankful.
no I didn't. as mentioned earlier RNA-seq library is of 150/200bp size so miRNA can't be captured by this as.