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Can I compute Tumour Mutational Burden from rnaseq fastq files?

Hello,

I am not familiar with tumour mutational burden, but I was asked to obtain it from fastq files sequenced with RNA-Seq. How can I do it with this data? I think it would be better with exome sequencing though.

Thank you

tmb rna-seq snp vcf
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written by Pin.Bioinf Pin.Bioinf

1 answer

check this post : Using RNA-Seq to identify non-synonymous mutational load

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written by Nicolas Rosewick Nicolas Rosewick

Thanks a lot! Actually I saw that post but did not know mutational load was similar to mutational burden. Thanks

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written by Pin.Bioinf Pin.Bioinf

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