Question about primer designing in case of mines strand of DNA
Hi. i'm going to design primer for qPCR analysis. But the Nucleotide sequence is mines (antisense strand). Do I need to make the sequence reverse complement before designing the primer? Thank you
• 1,209 views
•
link
written
by
m.pezhmanmehr •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
I am trying to get my head around amplicon sequencing library prep but can't understand how the ada…
written by ScottDansk •
-
Extracting Promoter Sequence from reverse Strand using NCBI Entrez and Eukaryotic Promoter Database?
written by Lys •
-
Is first strand the sense or the antisense?
written by Jay-Run •
-
NCBI Primer-BLAST primers
written by ovariohisterectomia •
-
Tool to design Q-PCR primers with exon skipping?
written by gabriel.monteiro233 •
-
Are there any aligners that enable me to align sense strands only to a sense strand reference seque…
written by b10hazard •
-
Primer sequences after PCR, Assembly and PCR
written by s.kyungyong64 •
-
Reverse complement of selective sequences in a maf file
written by RoryC •
-
Bowtie2 local alignment SAM file SEQ output - reverse complement or not ?
written by Roger •
-
Genbank file format is wrong?
written by eddie.im
