Hi All,
I am currently having a data from TCGA LUSC samples. I am planning to conduct differential gene expression analysis between normal vs. Tumor samples. My sample size is,Tumor = ~ 200 and Normal < 10. My question is, can we compare 10 Normal samples with 200 Tumor samples? is that statistically correct or do I get correct results? Is that I am thinking wrong?
Secondly, can I pool in Normal samples from other cancer types to increase the sample size for Normal types? in what context TCGA says a sample as normal?
I might be very stupid in asking this, but I want to put my thoughts at rest. Thanks a lot for your assistance, it is highly appreciated.
Sincerely,
Dave,
Can we compare 3 normal with 300 tumor sample? CESC in TCGA has only 3 normal samples.
Yes, 3 samples is the absolute minimum for finding group-level differences.
great! Thank you very much.
Thanks a lot, I am clear now.