pacbio-illumina hybrid assemblies
We are looking into assembly with pacbio and illumina data, and wondering if there are any tools or well-validated workflows for hybrid assemblies, as well as any recommendations for coverage. this is for a eukaryotic genome. additionally, has anyone ever tried a pacbio-10XGenomics hybrid assembly?
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This guy seems to be trying it with quickmerge.
https://github.com/mahulchak/quickmerge/issues/22
It would be good to use the advantage of the 10x supernova assemblies and not just feed them as simple illumina data to another assembler...
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https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/Large-Genome-Assembly-with-PacBio-Long-Reads