from coordinate to motif
Hi, Does anyone know if there exists such a database who can return the possible motifs when I input the coordinate(chr,start,end)?
Thank you in advance!
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2 answers
For individual motifs, I prefer fimo from the MEME suite. It accepts fasta format. Bedtools getfasta can convert coordinates to fasta. Motif PFMs can be downloaded from JASPAR.
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You could make your own database with FIMO and query it as needed:
Once you have such a resource, it is easy to query on an ad-hoc basis:
$ echo -e "chrN\t1234\t5678" | bedmap --echo --echo-map --delim '\t' - fimoResults.bed > answer.bed
Or:
$ bedmap --echo --echo-map --delim '\t' myRegions.bed fimoResults.bed > answer.bed
FIMO results generally include the sequence of the TF model, but you can export to samtools faidx for getting back specific sequence over the wider region.
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There are several, that perform motif analysis. on genomic regions
HOMER MEME ChIPSeek