Hello I think I have a quick question:
I received a demo dataset of fastq rna-seq data: My assignment is to find out from which organism they are. I tought of using BLAST to perform this task. I followed the biostar handbook, first converting the fastq sequences to fasta, and then using the BLAST web tool.
When entering my file with 100k sequences, it will return an error. When feeding it my first 5 sequences, I get the results for the first one, and I can then choose the results for the next files from the drop down menu.
So I understand it will present me the results for just a copule of sequences. But is there a functionality that will evaluate the results of all 100k sequences?
I don't really know if I should make an assembly, or whether I should invest in running the blast locally, and then looking at the organisms of the #1 result from each blast...?