StringTie giving different results - same dataset
Hello,
I made two transcript assemblies with the same data set (same bam files) with StringTie, and I am getting somehow different results. Both times I got 223,641 transcripts in total, but the first time I ran it I got around ~40,000 de novo transcripts, and the second I got ~50,000.
I would like to know why. What is changing or is random in the algorithm?
Thank you,
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Set a seed and try the pipe line. If you are still getting different numbers then contact the devs
Hi cpad0112
Can you clarify how can we set seed? This sounds something new for me as well.
Do you know how to set a seed by any chance?