Interpreting ATAC-seq alignment results
I have two ATAC-seq data samples which I aligned to reference genome hg19 using STAR aligner. How do I judge the quality of the data? I am new to ATAC-sequencing and understanding the data quality. The mapping results are as below: **
- Sample 1:
* Tags mapped %: 22.32 Multi-mapped reads %: 1.23 Mapped duplicates %: 5.44 Tags unmapped %: 71.01 *
- Sample 2:
** Tags mapped %: 41.80 Multi-mapped reads %: 2.90 Mapped duplicates %: 13.43 Tags unmapped %: 41.88
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