Hi, I had performed RNA-seq alignment using STAR for the sample Solanum tuberosum . I didn't understand the parameter "Number of splices: Annotated (sjdb)" and as it is zero for my sample I am pretty much worried if the gff file is used or not during analysis. Can anyone give me an explanation on this parameter? For reference I have attached the Alignment stats below.

                         Started mapping on |   Apr 21 17:22:19
                                Finished on |   Apr 21 17:28:05
   Mapping speed, Million of reads per hour |   151.85

                      Number of input reads |   14594080
                  Average input read length |   298
                                UNIQUE READS:
               Uniquely mapped reads number |   12744712
                    Uniquely mapped reads % |   87.33%
                      Average mapped length |   294.75
                   Number of splices: Total |   12412944
        Number of splices: Annotated (sjdb) |   0
                   Number of splices: GT/AG |   12183896
                   Number of splices: GC/AG |   113682
                   Number of splices: AT/AC |   3944
           Number of splices: Non-canonical |   111422
                  Mismatch rate per base, % |   0.91%
                     Deletion rate per base |   0.06%
                    Deletion average length |   2.90
                    Insertion rate per base |   0.05%
                   Insertion average length |   3.01
                         MULTI-MAPPING READS:
    Number of reads mapped to multiple loci |   1364700
         % of reads mapped to multiple loci |   9.35%
    Number of reads mapped to too many loci |   98772
         % of reads mapped to too many loci |   0.68%
                              UNMAPPED READS:

Number of reads unmapped: too many mismatches | 0 % of reads unmapped: too many mismatches | 0.00% Number of reads unmapped: too short | 383412 % of reads unmapped: too short | 2.63% Number of reads unmapped: other | 2484 % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00%