Hi,
I have about 57 BED files with various overlapping intervals between them. I would like to merge them all, like with multiple intersect from BEDTools, but average the score associated with the overlap across the files. I see there is a function in MergeBED to add an additional column to average the score from overlapping intervals, but I don't see this for 'multiple intersect' for multiple files.
The caveat is my limited programming knowledge, so I use the Galaxy server. Is there a way to do this?
Thanks
To do this efficiently, you can use bedops and bedmap to calculate this over N files (N=57, in your case).
$ bedops -u A.bed B.bed ... N.bed > union.bed
$ bedops -m A.bed B.bed ... N.bed > merge.bed
$ bedmap --echo --mean --delim "\t" merge.bed union.bed > answer.bed
The file answer.bed will contain merged intervals and the arithmetic mean of signals in overlapping intervals from files A.bed through N.bed (these are your 57 BED5 files, where the fifth column contains signal, per UCSC convention).
More documentation at: http://bedops.readthedocs.io/en/latest/
Galaxy is great, but there's no real substitute for the Unix command line. Learn it if you're doing informatics.
Thanks for this. However, I am unfamiliar with these programs and how to use them. I see they are not listed on the Galaxy server, is there an online version of these tools to upload the files for processing? Thanks
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Thanks for this. However, I am unfamiliar with these programs and how to use them. I see they are not listed on the Galaxy server, is there an online version of these tools to upload the files for processing? Thanks
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