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Multiple alignment to matrix generation

I am using Python 3.3+ version and I want to use an multiple sequence aligned file (Clustalw format) to generate a distance-based matrix. Since I'm still on learning stages with python, I want to know how do I use the aligned sequences to generate a matrix (by giving my own values for amino acid frquencies).

matrix pylogenies

Though the questions already exist but I don't find the helpful answers, can any guidelines be provided?

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