Is it practical to trap miRNA using PCR?
Dear Biostars, Hi
Q: I wonder if it is practical to check the presences of exact miRNAs using PCR (or any other simple and cheap method)?
I was thinking that because miRNA are short, designing appropriate PCR primers should be difficult.
Thanks!
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how is it related to bioinformatics ?
There are methods that allow direct ligation of adapters to small RNA (https://genohub.com/small-rna-sequencing-library-preparation/ ). You may be able to use one of those with PCR instead of sequencing (not my area of expertise). Posting this on SeqAnswers.com may be useful.
Hello Farbod!
We believe that this post does not fit the main topic of this site.
Not related directly to bioinformatics.
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!
Hi, Sorry about that. I was thinking about some sort of in silico PCR or digital PCR or something like that and because of that I asked it here. but you are right. Thanks