Assembling my genome
Hi all, I'm actually trying to assemble my genome. I have 2 fasta file: reads1.fq and reads2.fq
Those file are comming from an illumina Hiseq 3000 150bp
And I would like to assemble my genome but I do not realy know programme to do it. I would like if possible a programme easy to use with a good documentation, does someone have an idea of such a program?
Thanks a lot
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There are couple of program to perform the assembly, but before that , let us know the organism the data belong to. In the mean time you can also look at this interesting post.
It is from a butterfly specie with a genome size aroud 1.49 Gb
Have you tried using Google before asking this question?
What organism? Are there reference sequences? Do you want to de novo assemble?
These are questions you need to answer to get an idea if a particular assembler will suit your needs.
A few popular ones off the top of my head, though I can't attest to their ease of use or documentation:
MIRA, SPAdes, Velvet, SOAP...
<30 seconds on google returns this:
https://en.wikipedia.org/wiki/De_novo_sequence_assemblers#Commonly_used_programs