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How to perform KaKs_Calculator from SNPEff's output

Hi, all,

I have obtained the annotated SNP (VCF file) by SNPEff. How to calculate ka/ks using KaKs_Calculator or other programs.

rna-seq
  1. KaKs_Calculator requires sequences in the .axt format not SNPs.
  2. What is the goal of using KaKs_Calculator?

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