This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Plotting Pearson correlation between two expression matrices

Hi,

I have two matrices; one from bulk RNA-seq and the other from single cell seq. Based on this figure, if I am not wrong, I must firstly normalize each matrix with RPM method, but I did not figure out how to produce such a plot for two normalized matrices

https://ibb.co/cokYrJ

rna-seq r single cell seq

sorry my image was not visible?

By following the guide linked by @Wouter images you upload to imgbb.com can be parsed automatically by Biostars web server so they actually show in your post. People do not need to click any links.

Here is your image for example:

Untitled

Normalization is irrelevant for that, it looks like it's just plotting total UMI counts vs. read counts in the pseudo-bulk sample.

But why said the (Read per million) in X axis?

RPM just changes the scale, it's not needed.

0 answers

No answers yet.

Log in to answer this question.