Idea about increasing coverage in sequencing
I likely need a 100x increase in the number of reads per Amplicon (using amplicon library) . Can you guys provide ideas of doing that? One option could be to create a new amplicon library with 1/10 the number of amplicons but still need to get the additional 10x improvement. Any idea?
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vkgaur25 •
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I am not sure if this is directly related to bioinformatics but here goes.
Since you sequence a small fraction of the library that gets made you can do multiple lanes of additional sequencing to reach any target read number. Note: You would be sampling more of the same library.
Also, don't fall into the naturally 'human' illusion that higher depth of coverage equates to better quality data. It greatly depends on your experiment, but you have not elaborated on what you are actually doing.
Why do you need so much coverage?